文献名： Comparative Toxic Effects of Manufactured Nanoparticles and Atmospheric Particulate Matter in Human Lung Epithelial Cells

上海弘升科技發展有限公司： Yun Wu¹  , Mei Wang¹, Shaojuan Luo², Yunfeng Gu¹, Dongyang Nie³, Zhiyang Xu¹, Yue Wu¹, Mindong Chen¹ and Xinlei Ge¹

¹  Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control (AEMPC), Collaborative Innovation Center of Atmospheric Environment and Equipment Technology (AEET), School of Environmental Science and Engineering, Nanjing University of Information Science & Technology (NUIST), Nanjing 210044, China

²  School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China

³  School of Atmospheric Sciences, Nanjing University, Nanjing 210023, China

摘要：Although nanoparticles (NPs) have been used as simplified atmospheric particulate matter (PM) models, little experimental evidence is available to support such simulations. In this study, we comparatively assessed the toxic effects of PM and typical NPs (four carbonaceous NPs with different morphologies, metal NPs of Fe, Al, and Ti, as well as SiO₂ NPs) on human lung epithelial A549 cells. The EC50 value of PM evaluated by cell viability assay was 148.7 μg/mL, closest to that of SiO₂ NPs, between the values of carbonaceous NPs and metal NPs. All particles caused varying degrees of reactive oxygen species (ROS) generation and adenosine triphosphate (ATP) suppression. TiO₂ NPs showed similar performance with PM in inducing ROS production (p < 0.05). Small variations between two carbonaceous NPs (graphene oxides and graphenes) and PM were also observed at 50 μg/mL. Similarly, there was no significant difference in ATP inhibition between carbonaceous NPs and PM, while markedly different effects were caused by SiO₂ NP and TiO₂ NP exposure. Our results indicated that carbonaceous NPs could be served as potential surrogates for urban PM. The identification of PM model may help us further explore the specific roles and mechanisms of various components in PM.  

关键词：atmospheric particulate matter; nanoparticles; A549 cells; cytotoxicity